Journal: Kidney International Reports

Article Title: Markers of Mineral Metabolism in Children With CKD Stages 2 to 5D

doi: 10.1016/j.ekir.2026.103800

Figure Lengend Snippet: Markers for chronic kidney disease–mineral and bone disorder in children with CKD according to CKD stages. Values for (a) phosphate, (b) iFGF23, (c) total FGF23, and (d) iPTH are given as age- and sex-related z-scores. For patients on dialysis treatment, estimated glomerular filtration rate was set to 10 ml/min per 1.73 m 2 . Gray shaded areas indicate the normal range (mean ± 2 SD), with the 0-line highlighted in white. #, ##, ### and #### indicate statistical significance from healthy controls at P < 0.05, P < 0.01, P < 0.001, and P < 0.0001, respectively (1-sample t test or Wilcoxon signed rank test according to Kolgorow-Smirnov normality test). Values not sharing superscript letters a, b, c, and d are significantly different from the values for other CKD stages (1-way analysis of variance, followed by Tukey’s multiple comparisons, or Kruskal-Wallis-test, followed by Dunn’s multiple comparisons). CKD, chronic kidney disease; FGF23, fibroblast growth factor 23; iFGF23, intact FGF23; iPTH, intact parathyroid hormone.

Article Snippet: Enzyme-linked immunosorbent assay kits were used for quantitative determination of iFGF23 (Quidel, Catalog # 60-6600, RRID: AB_2891250 ), total FGF23 using the C-term FGF23 assay kit, which measures both the full-length hormone and its posttranslationally cleaved C-terminal fragments (Quidel, Catalog # 60-6100, RRID: AB_2722648 ), sKlotho (Immuno Biological Laboratories, Catalog # 27998, RRID: AB_2750859 ) in plasma, and sclerostin (TECO Medical, Catalog # TE1023-HS, RRID: AB_2894880 ) in serum.

Techniques: Filtration

Journal: Kidney International Reports

Article Title: Markers of Mineral Metabolism in Children With CKD Stages 2 to 5D

doi: 10.1016/j.ekir.2026.103800

Figure Lengend Snippet: Markers for chronic kidney disease–mineral and bone disorder in children with chronic kidney disease as a function of eGFR. Values for (a) phosphate, (b) calcium, (c) sclerostin, (d) 25(OH)D, (e) total FGF23, (f) iFGF23, (g) sKlotho, (h) iPTH, (i) 1,25(OH) 2 D 3 , and (j) AP are given as age- and sex-related z-scores. For patients on dialysis treatment, eGFR was set to 10 ml/min per 1.73 m 2 . The respective best-fit function (sigmoidal, linear, or semilogarithmic) is presented, with the blue-shaded area indicating the 95% confidence interval. Gray shaded areas indicate the normal range (mean ± 2 SD), with the 0-line highlighted in white. 1,25(OH) 2 D 3 , 1,25-dihydroxy vitamin D 3 ; 25(OH)D, 25-hydroxyvitamin D3; AP, alkaline phosphatase; eGFR, estimated gloemrular filtration rate; FGF23, fibroblast growth factor 23; iFGF23, intact FGF23; sKlotho, soluble Klotho; iPTH, intact parathyroid hormone.

Article Snippet: Enzyme-linked immunosorbent assay kits were used for quantitative determination of iFGF23 (Quidel, Catalog # 60-6600, RRID: AB_2891250 ), total FGF23 using the C-term FGF23 assay kit, which measures both the full-length hormone and its posttranslationally cleaved C-terminal fragments (Quidel, Catalog # 60-6100, RRID: AB_2722648 ), sKlotho (Immuno Biological Laboratories, Catalog # 27998, RRID: AB_2750859 ) in plasma, and sclerostin (TECO Medical, Catalog # TE1023-HS, RRID: AB_2894880 ) in serum.

Techniques: Filtration

Journal: Kidney International Reports

Article Title: Markers of Mineral Metabolism in Children With CKD Stages 2 to 5D

doi: 10.1016/j.ekir.2026.103800

Figure Lengend Snippet: Alterations in the ratio of iFGF23 to (a) phosphate (Pi) and (b) calcium (Ca) in pediatric patients with chronic kidney disease as a function of eGFR. For patients on dialysis treatment, eGFR was set to 10 ml/min per 1.73 m 2 . Data are given as age- and sex-related z-scores. The respective best-fit function (semi-logarithmic) is presented, with the blue-shaded area indicating the 95% confidence interval. Gray shaded areas indicate the normal range (mean ± 2 SD), with the 0-line highlighted in white. eGFR, estimated gloemrular filtration rate; iFGF23, intact fibroblast growth factor 23.

Article Snippet: Enzyme-linked immunosorbent assay kits were used for quantitative determination of iFGF23 (Quidel, Catalog # 60-6600, RRID: AB_2891250 ), total FGF23 using the C-term FGF23 assay kit, which measures both the full-length hormone and its posttranslationally cleaved C-terminal fragments (Quidel, Catalog # 60-6100, RRID: AB_2722648 ), sKlotho (Immuno Biological Laboratories, Catalog # 27998, RRID: AB_2750859 ) in plasma, and sclerostin (TECO Medical, Catalog # TE1023-HS, RRID: AB_2894880 ) in serum.

Techniques: Filtration

Journal: Kidney International Reports

Article Title: Markers of Mineral Metabolism in Children With CKD Stages 2 to 5D

doi: 10.1016/j.ekir.2026.103800

Figure Lengend Snippet: Overview of the dynamic changes of 8 key markers for chronic kidney disease– mineral and bone disorder in pediatric patients with chronic kidney disease as determined in the present study, as a function of eGFR. Values for iFGF23, iPTH, phosphate, 25(OH)D, sKlotho, calcium, 1,25(OH) 2 D 3 , and sclerostin are given as age- and sex-related z-scores. For patients undergoing dialysis, eGFR was set to 10 ml/min per 1.73 m 2 . The respective best-fit function (sigmoidal, linear, or semilogarithmic) is presented, with the blue shaded area indicating the 95% confidence interval. Gray shaded areas indicate the normal range (mean ± 2 SD), with the 0-line highlighted in white. 1,25(OH) 2 D 3 , 1,25-dihydroxy vitamin D 3 ; 25(OH)D, 25-hydroxyvitamin D3; eGFR, estimated gloemrular filtration rate; iFGF23, intact fibroblast growth factor 23; iPTH, intact parathyroid hormone; sKlotho, soluble Klotho.

Article Snippet: Enzyme-linked immunosorbent assay kits were used for quantitative determination of iFGF23 (Quidel, Catalog # 60-6600, RRID: AB_2891250 ), total FGF23 using the C-term FGF23 assay kit, which measures both the full-length hormone and its posttranslationally cleaved C-terminal fragments (Quidel, Catalog # 60-6100, RRID: AB_2722648 ), sKlotho (Immuno Biological Laboratories, Catalog # 27998, RRID: AB_2750859 ) in plasma, and sclerostin (TECO Medical, Catalog # TE1023-HS, RRID: AB_2894880 ) in serum.

Techniques: Filtration

Journal: Kidney Medicine

Article Title: A Large Deletion With a Large Impact: Homozygous 5,600 bp Deletion of the GALNT3 Gene Causing Hyperphosphatemic Tumoral Calcinosis

doi: 10.1016/j.xkme.2026.101241

Figure Lengend Snippet: Impact of GalNAc-T3 enzyme dysfunction; normal situation (left), deletion leading to GalNAc-T3 dysfunction (right). FGF-23, fibroblast growth factor 23; FGFR, fibroblast growth factor receptor.

Article Snippet: Intact FGF23 was measured with the human intact FGF23 enzyme-linked immunosorbent assay (#60-6600, Lot 173373, Quidel Corporation) according to the manufacturer’s protocol.

Techniques:

Journal: JBMR Plus

Article Title: Multi-tissue spatial transcriptomics identified simultaneous responses to oxidative stress and apoptosis in parallel with tissue-specific reprogramming in modeled chronic kidney disease-mineral and bone disorder

doi: 10.1093/jbmrpl/ziaf161

Figure Lengend Snippet: Spatial transcriptomics identified tissue subtypes in muscle and bone. (A-C) Plasma intact FGF23 and serum TNF and blood urea nitrogen (BUN) levels in control (Ctrl) and CKD-MBD (CKD) mice (* p < .05; ** p < .01; *** p < .001 vs controls). H&E bone-muscle cross-sections for Ctrl (D) and CKD (E) and the Seurat clusters mapped on the sections (F and G). (H) Tissue and fiber type specific marker gene expression. (I) UMAP of Ctrl and CKD samples, with major tissue and muscle fiber types labeled. (J) Localization of major muscle fiber types in Ctrl and CKD tissue sections (colors correspond to UMAP in H).

Article Snippet: Plasma FGF23 was assessed using a rodent specific commercial ELISA for bioactive, intact FGF23 (“iFGF23”; Quidel, Inc.).

Techniques: Clinical Proteomics, Control, Marker, Gene Expression, Labeling

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Effect of CRISPR/Cas9-induced Vps13a knockdown on FGF23 production. ( A ) Fgf23 mRNA ( n = 7) expression normalized to β-actin ( Actb ) in control cells transfected with non-targeting (NT) sequence and Vps13a knockdown (KD) cells using CRISPR/Cas9 system. ( B ) C-terminal FGF23 protein concentration ( n = 8) in the cell culture supernatant of NT and Vps13a knockdown cells. All data are shown as arithmetic means ± SEM. (A: Unpaired Student’s t -test; B: Mann-Whitney U test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: CRISPR, Knockdown, Expressing, Control, Transfection, Sequencing, Protein Concentration, Cell Culture, MANN-WHITNEY

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Knockdown of Vps13a influences mRNA expression of FGF23 regulators . Relative mRNA expression of Phex ( A , n = 6) and Galnt3 ( B , n = 8) mRNA normalized to β-actin ( Actb ) in non-targeting (NT) control cells and Vps13a knockdown (KD) cells. All data are shown as arithmetic means ± SEM. (A: Unpaired t -test with Welch’s correction; B: Unpaired Student’s t -test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: Knockdown, Expressing, Control